2022-09-29
Genomic DNA Isolation from Plant Tissue
Genomic DNA was isolated from plant tissue by freezing a small leaf piece in liquid nitrogen, grinding the tissue in a microtube, and adding DNA extraction buffer. The mixture was vortexed and centrifuged, and the supernatant was transferred to a new tube. Isopropanol was added to the supernatant to precipitate the DNA. After centrifugation, the pellet was washed with ethanol and air-dried before being dissolved in sterile water. The solution was briefly heated and spun down, and a small volume was set aside for PCR.
The content and copyright of the experimental protocol belong to Ghent University.
Under no circumstances should any information be used for commercial purposes.
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