2020-11-19
Callus Induction of Cotyledons of Arabidopsis thaliana
Plants can thrive in hydroponic systems, where their roots are suspended in nutrient solutions instead of soil. Essential minerals, sucrose, organic substances, and vitamins are added to these solutions to support growth. This technology allows for the cultivation of excised plant parts and facilitates the formation of undifferentiated callus tissue.
Placed the cotyledons on a nutrient-rich in vitro growth medium.
Arabidopsis thaliana was chosen for this experiment due to its short generation time (6 weeks), high seed production capacity (over 10,000 seeds), self-fertilization ability, and adaptability to sterile, defined media. Auxins and cytokinins are essential for plant growth and callus formation.
Successful callus formation
Yellow circles denote bacterial contamination preventing callus formation.
Failed callus formation and plant death
The content and copyright of the experimental protocol belong to Ghent University.
Under no circumstances should any information be used for commercial purposes.
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